Expert did his Ph.D. research on the role of lipopolysaccharides (LPS) on cell-to-cell signaling and motility of Myxococcus xanthus bacteria. Emphasis was on the role of oliosaccharides associated with cell receptors in prokaryote organisms. He also developed a fermentation process to produce calcium magnesium acetate (CMA) from xylose and dolime using thermophilic Baciluus sp. CMA is a potential road salt replacement. He developed a conduction calorimetry method to monitor the fermentation reaction.
He co-founded Custom Gene Products, a business structured to produce recombinant proteins in the Baculovirus System. The virus is genetically engineered to contain DNA coding for the desired protein. Insect cells are infected and the recombinant virus is purified by plaque assays. Insect cells are infected with the purified virus and the recombinant protein is expressed, isolated, and purified from the insect cell paste.
His PhD research utilized high performance liquid chromatography (HPLC), gel electrophoresis (PAGE), and NMR to characterize the monosaccharides in the LPS core and O-antigen structures. He characterized the polysaccharide components of LPS using monoclonal antibodies. He is an expert in sequencing polysaccharides, electrophoresis, Western blotting, and bacterial fermentations.
He co-founded a business designed to consult with industry in the areas of quality systems, management systems, manufacturing operations, and warehouse operations. The company specialized in the citrus industry where they conducted training seminars covering quality applications in the preparation of orange juice.
He integrated a vision system to direct a robotic work cell which placed odd components on printed circuit boards. He has worked with computer vision systems in robots and is knowledgeable about pick and place robots.
He designed and constructed a fried and frozen foods pilot plant and a fluid bed dryer/reactor pilot plants.
He directed a R&D team of 21 researchers for Ecolab's consumer products division. The organization developed and launched five new cleaning products. He directed a program to improve the cost and quality of tripolyphosphate raw material saving over $5 million per year. He managed work to develop contract co-packers, raw material suppliers, and internal manufacturing facilities. He was responsible for the safety and regulatory aspects of the products. Expert directed an encapsulation project that resulted in a patent for an encapsulation process allowing the use of chlorine bleach and enzymes simultaneously in the same cleaning product. He developed low-dust enzyme carriers via coating and encapsulation.
He directed a Clorox process development organization consisting of 25 engineers and technicians. They were involved in laundry, specialty cleaning, food, and charcoal products. He developed a process for producing peracid bleaches in collaboration with SRI. He managed a process development project to produce percarbonate bleach.
He is cofounder of Expert's Firm a company organized to develop, produce, and market, biocatalyist and biomaterials. The biocatalytic materials are based on technology developed at the University of Minnesota. Expert's Firm, has exclusive rights to a recent patent application. He was a consultant to the University during the research and is named on the patent application. He started his career as a process development engineer for the Procter and Gamble International Division. His assignments included plant startups in Mexico, Peru, Venezuela and the Philippines. The manufacturing processes included spray drying, enzyme agglomeration and surfactant production.