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 Expert  107708

Expert in Protein Biochemistry, Purification, Characterization; Gentle Immunoaffinity Chromatography

Available for your Consulting and Expert Witness Needs

Wisconsin (WI)
Education Work History Career AccomplishmentsPublicationsConsulting Services Expert Witness

Summary of Expertise: Listed with other top experts in: 
Expert is internationally known for his contributions to the fields of biochemistry and molecular biology. He has worked in this area since 1964, generally in purifying enzymes involved in RNA synthesis, characterizing the structure and function of enzymes, and studying protein-protein and protein-DNA interactions.

Expert's lab studies RNA polymerases and transcription factors from bacteria (especially E. coli) and from eukaryotes including wheat, yeast, calf thymus, and human cells. He discovered the complex subunit structure of E. coli RNA polymerase and the initiation factor, sigma-70, responsible for specific RNA synthesis (transcription). He also studies the mechanism of action of the phytotoxin tagetitoxin in inhibiting RNA polymerase.

Expert teaches a 28-lecture course at the Expert's University at Madison and a two week lab course at Cold Spring Harbor. He organized the Keystone Symposia on protein purification in 1986 and 1992. He has developed the use of polyethyleneimine precipitation as an important step in clarifying extracts and fractionating proteins. He has helped popularize the use of glycerol and dithiothrietol as protein stabilization agents.

As an early user of polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE, Expert developed techniques for improving staining and quantitation of protein in gels. He dramatically improved the efficiency of eluting microgram amounts of enzymes from SDS gels, removal of SDS, and renaturation of active enzymes.

Since a sabbatical in 1983-84 with a medical diagnostic company, Expert has been very active in producing MAbs as reagents to aid in his biochemical studies. He has developed methods for rapid epitope-mapping. He has recently developed and patented a method for screening for rare MAbs ideal for immunoaffinity chromatography. These "polyol-responsive" MAb columns bind enzymes tightly but release under very gentle conditions of salt and polyol (glycerol, ethylene glycol, propylene glycol). This allows rapid immunoaffinity purification of labile enzymes.

Expert has considerable experience isolating enzymes from natural sources such as bacterial, yeast, wheat germ, and mammalian cell extracts. He is also very experienced at overproducing enzymes expressed from genes cloned into E. coli, solubilizing insoluble enzymes found in inclusion bodies, and refolding to yield active enzymes.

Expert has focused his career on understanding the RNA synthesis or transcription machinery. This includes RNA polymerases and transcription factors from prokaryote (mainly E. coli) and eukaryotes (wheat, yeast, calf, human). His work has involved purification, subunit characterization, immunochemical analysis, cloning and overproducing subunits, and protein-protein crosslinking.

In an area of secondary expertise, Expert has used heterobifunctional crosslinking reagents to determine where on RNA polymerase certain RNA polymerase-binding transcription factors are binding and thus to infer adjacency and subunit architecture.

Expert has used monoclonal antibodies to aid in biochemical studies of enzymes. He has developed several new ways of epitope mapping that are faster and easier than traditional protein chemical approaches.




molecular biology


gene expression


gene expression regulation


protein purification


protein purification method


enzyme purification


gel electrophoresis


monoclonal antibody production


affinity chromatography




enzyme production


RNA polymerase


protein-protein crosslinking




epitope mapping

Show Secondary and Basic Areas of Expertise
Expert may consult nationally and internationally, and is also local to the following cities: Milwaukee, Wisconsin;  Madison, Wisconsin;  Kenosha, Wisconsin;  Racine, Wisconsin;  Rockford, Illinois;  Aurora, Illinois;  Elgin, Illinois;  Waukegan, Illinois;  Arlington Heights, Illinois;  and Schaumburg, Illinois.

Often requested
with this expert:

Protein Biochemistry, Glycoproteins
Protein Biochemistry, E Coli Mutation, Thioredoxins, Amino...
Biopharmaceutical Development

Year   Degree   Subject   Institution  
1969   Ph.D.   Biochemistry and Molecular Biology   Harvard University (with renowned genetic scientist, Professor James D. Watson)  
1964   B.S.   Chemistry   Caltech  

Work History:
Years   Employer   Department   Title   Responsibilities

1971 to


(Undisclosed University)



Professor Emeritus


He runs a small laboratory, teaches and participates in departmental and university activities.

1983 to 1984


Genetics Systems, Inc




Visiting Director of Research


Expert spent a year on a Guggenheim Fellowship in a monoclonal antibody company in Seattle WA, where he built the biochemistry division.

Career Accomplishments:

AACR, Protein Society, ACS, ASBMB

Professional Appointments

Expert is a member of many professional societies, is currently Editor-in-Chief of the journal, Protein Expression and Purification. He is also a series editor for a series of biotechnical resource manuals published by Oxford University Press.


Fellow of American Academy of Microbiology and AAAS, Waksman Medal, Pfizer Award, Honorary Degree from Watson School of Biological Science, Cold Spring Harbor Laboratory

Publications and Patents Summary

He has over 240 publications and about 8 patents

Selected Publications and Publishers  
 - Genome Research  
 - Methods in Enzymology  
 - Analytical Biochemistry  
 - Journal of Biological Chemistry  

Consulting Services:
Selected Consulting Examples:
  • He acted as expert witness in three biotechnology-related patent cases, in the area of protein biochemistry.
  • He is a member of the Scientific Advisory Board for a local monoclonal antibody production company.
  • He has consulted for several investment companies in the areas of biotechnology and technology evaluation.
  • He has on numerous occasions consulted with biotechnology companies in the area of protein purification.
Recent Client Requests:
  • Expert in in biologics manufactory to facilitate process development and optimization for mammalian cell colure.
  • Protein purification expert for consulting on validation of novel protein purification technique.
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Expert Witness Experience Summary:
Expert has served three times as an expert witness in the area of protein biochemistry, including expert report preparation, deposition, and testimony
Recent Litigation Client Requests:
  • Antibody purification expert witness for consulting on Patent analysis, prior art search.
  • Expert in protein biochemistry for expert opinion on a technical detail of patent interference lawsuit.
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.

International Experience:
Years   Country / Region   Summary
to   Russia   Expert has lectured internationally, including six lectures in Russia as an invited guest of the Soviet Academy of Science.
1969 to 1971   Switzerland   He was a postdoctoral Fellow at the Institute for Molecular Biology in Geneva
2012 to 2012   Germany   He was an instructor in an EMBO course on Protein Purification and Characterization in Hamburg Germany for 10 days

Additional Skills and Services:

Expert has taught Protein Purification at Cold Spring Harbor for the last 22 years and his course in Madison since 1986.

Supplier and Vendor Location and Selection

Expert founded the UW Biotechnology Center in 1984 and stimulated the growth of the Biotech business community in Madison WI from 3 in 1984 to over 150 in 2009. He also has been an active angel investor, consultant in start-ups and founded the antibacterial company, ConjuGon, in 2001 in Madison


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