ORC Expert Advisory Services ORC International

  home    |    find an expert    |    request an expert    |    join our network    |    about us     expert login  
Expert Consulting and Expert Witness Services
  search tips 
Share this page:  Send to LinkedIn Send to Facebook Tweet This Email Print

 Expert  714289

Expert in Protein Biochemistry, E Coli Mutation, Thioredoxins, Amino Acid Residues, etc.

Available for your Consulting and Expert Witness Needs

Minnesota (MN)
Education Work HistoryPublicationsConsulting Services Expert Witness Market Research

Summary of Expertise:

Listed with other top experts in: 

Expert has expertise in the purification and characterization of disulfide-containing redox proteins from a variety of micro-organisms. She has used the standard techniques of both low and high pressure chromatography on ion exchange and size exclusion gels. She is also familiar with affinity and immunochemical techniques. She is proficient with molecular biology procedures to isolate and clone genes and overproduce proteins of interest for both physical characterization and sequencing.

In her work, Expert routinely uses various types of chromatography including ion exchange, size exclusion, affinity, and hydrophobic variants and HPLC for purification. Protein characterization is done by gel electrophoresis, UV-visible spectroscopy, and fluorescence. She has done both steady-state and rapid kinetics to correlate enzyme structure with function.

Expert and her colleagues are interested in producing mutant proteins by random and site-directed mutagenesis. They have used both kinetic data and molecular modeling to try and predict the effects of amino acid substitutions on structure.

Several years ago, Expert initiated a project on the isolation and purification of an herbicidal natural product from the blue-green alga, Scytonema hofmanni. She and her colleagues were able to characterize the compound using mass and infrared measurements.

Expert has an active program in collaboration with another faculty member to isolate and identify natural products from freshwater algae and plants. Her current work is on anti-algal compounds and an insect attractant produced by Eurasian water milfoil. Bioassays are set up with the appropriate test organisms and standard techniques are used to extract small, active molecules from plant materials such as thin-layer and high-pressure chromatography.

Expert has been investigating the mechanism of action for a disulfide-containing redox protein called thioredoxin for several years. In collaboration with colleagues at the University of Arizona, she has used both steady-state and stopped-flow techniques to monitor interactions of thioredoxin with other proteins and to measure rates of electron transfer. These kinetic analyses have been coupled with protein engineering to identify amino acid residues in the protein which are critical for activity.




protein biochemistry


protein characterization


protein purification


protein structure




amino acid







Show Secondary and Basic Areas of Expertise
Expert may consult nationally and internationally, and is also local to the following cities: Minneapolis, Minnesota;  Saint Paul, Minnesota;  Rochester, Minnesota;  Burnsville, Minnesota;  Saint Cloud, Minnesota;  Eden Prairie, Minnesota;  Minnetonka, Minnesota;  and Eau Claire, Wisconsin.

Year   Degree   Institution  
1971   PhD   Biochemistry - University of Iowa  
1966   MS   Biology - Northwestern University  
1964   BS   Biology - Loyola University  

Work History:
Years   Employer   Department   Title  

1987 to


(Undisclosed University)


Department of plant biology



1974 to 1987


Gray Freshwater Biological Institute



Assistant Professor



Consulting Services:
Recent Client Requests:
  • Expert in commercial chromatography for consulting on anthocyanin pigment issues.
  • Protein purification expert for consulting on validation of novel protein purification technique.
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.

Market Research:
Her lab is also interested in natural products from cyanobacteria and the aquatic angiosperm, Eurasian water milfoil. Eurasian milfoil grows to nuisance levels in many American lakes. She and her class have found that it produces a low molecular weight compound that acts as an attractant to an insect which preferentially feeds and reproduces on the plant and is a potential biocontrol agent. They are now isolating and chemically characterizing this natural product. Further work is planned on its physiological function. They have also started a project to explore the distribution of algicidal and antibiotic natural products produced by freshwater cyanobacteria.

Click the green button above to contact Expert for a free initial screening call regarding your marketing research, industry research, and company research needs.  For research needs involving multiple experts or secondary research, a Research Director can be assigned to coordinate the work into a custom report for you as a potentially quicker and more cost effective alternative to doing this work yourself or hiring a boutique consultancy.  Remember, your initial screening call to speak with Expert is free.


Need the right expert?  We can help!

home       find an expert       request an expert       join our network       about us

Intota is now ORC Expert Advisory Services