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 Expert  720680

Expert in Biochemistry and Enzymology

Available for your Consulting and Expert Witness Needs

Ohio (OH)
Education Work History Career AccomplishmentsPublicationsConsulting Services Expert Witness

Summary of Expertise: Listed with other top experts in: 
A primary focus of Expert's research efforts has been the determination of the mechanism of action of enzyme-catalyzed reactions. He has applied a wide range of kinetic techniques, including steady-state and pre-steady-state studies, isotope effect studies, inhibition, inactivation, pH dependent, and temperature dependent studies, to a variety of bacterial, yeast, and mammalian enzyme systems.

enzyme kinetics


enzyme mechanism

The study of enzyme structure and enzyme mechanisms has remained the primary objective of Expert's research efforts. A wide range of experimental approaches have been used in his enzymological studies, ranging from techniques that are quite biological in nature (such as site-directed mutagenesis), to chemical techniques (such as chemical and active-site-directed modification studies), to physical and spectroscopic techniques (such as UV/visible, fluorescence, NMR, EPR, and X-ray crystallography.




The role of metal ions in biological systems and, in particular, the function of divalent cations in enzyme-catalyzed reactions remains an important area of interest. Studies in Expert's laboratory have ranged from the structural characterization of metal-amino acid and metal-peptide complexes, to metal binding plant lectins, to metal-activated and metalloenzymes. His work in this area has also included a study of the interaction of platinum anti-cancer drugs with nucleotides, and the formation of aluminum complexes of biological ligands.


Expert's research group has developed an extensive expertise in the purification of peptides and proteins. The experimental approaches that he has used range from classical techniques (such as gel filtration and ion exchange chromatography), to affinity techniques (such as hydrophobic chromatography, dye ligand chromatography, and specific ligand immobilization), to newer HPLC purification techniques.

protein purification


enzyme purification

Expert's research group is exploring methods for the selective introduction of unnatural amino acids into enzymes with the goal of altering their substrate specificity and modifying their catalytic efficiency.

enzyme engineering


enzyme modification


protein engineering

X-ray diffraction studies are a powerful tool to determine macromolecular structures at atomic resolution. The Ohio Crystallography Consortium at the Expert's University provides both instrumental capabilities and experimental expertise to solve protein structures. Expert's llaboratory is actively investigating the structures of a range of enzymes.

protein structure

Show Secondary and Basic Areas of Expertise
Expert may consult nationally and internationally, and is also local to the following cities: Detroit, Michigan;  Warren, Michigan;  Flint, Michigan;  Sterling Heights, Michigan;  Lansing, Michigan;  Ann Arbor, Michigan;  Livonia, Michigan;  Cleveland, Ohio;  Toledo, Ohio;  and Fort Wayne, Indiana.

Often requested
with this expert:

Chemical Engineering, Enzymes
Microbiology, Biotechnology, Cleanroom Microbiology,...
Biotechnology, Enzymes, Environmental Bioremediation,...
Recombinant Protein Expression, Purification, Cell-Based...

Year   Degree   Subject   Institution  
1976   PhD   Biochemistry   Pennsylvania State University  
1973   MS   Chemistry   Pennsylvania State University  
1967   BS   Chemistry   Fordham University  

Work History:
Years   Employer   Department   Title  

2000 to


(Undisclosed University)


Department of Chemistry




1989 to 2000


University of Akron


Department of Chemistry




1984 to 1989


University of Akron


Department of Chemistry


Associate Professor


1979 to 1984


Southern Illinois University


Department of Chemistry


Assistant Professor


1977 to 1979


University of Wisconsin


Department of Biochemistry


NIH Postdoctoral Fellow


Career Accomplishments:

Expert is a member of the American Chemical Society (ACS), the Protein Society, and the American Society of Biochemistry and Molecular Biology (ASBMB).

Publications and Patents Summary

He has 57 publications and two patents in biochemistry and enzymology.

Selected Publications and Publishers  
 - Journal of Biological Chemistry  
 - Adv. Enzymol.  
 - Journal of Molecular Biology  
 - IOS Press  
 - Biochemistry & Biophysics Research Communications  

Consulting Services:
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.


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