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 Expert  722747

Expert in Arthritis and Collagen Research


Available for your Consulting and Expert Witness Needs

Washington (WA)
USA
Education Work History Career AccomplishmentsPublicationsConsulting Services Expert Witness Market Research

Summary of Expertise:

Listed with other top experts in: 

Expert has expertise in and has established many animal models of disease at multiple institutions. These include models of human rheumatoid arthritis (RA) such as collagen-induced and adjuvant-induced arthritis in rodents. In addition, he has experience in models of osteoarthritis (OA) including spontaneous and collagenase-induced. In 1996, he established a company that develops and manufactures reagents and kits dedicated to arthritis research. He also has expertise in the rodent model of multiple sclerosis (EAE), the ovalbumin-induced asthma model, and rodent models of inflammatory bowel disease.
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animal disease model

Dr. Riefe has over 20 years of experience working in rheumatoid arthritis (RA) and osteoarthritis (OA) research. His expertise lies in the immuno- and pathobiology of these diseases with emphasis on the development of pre-clinical assays. These include animal models of RA such as collagen-induced (CIA) and adjuvant-induced arthritis in rodents and models of OA including spontaneous and collagenase-induced. He also has expertise in developing in vitro assays of disease including cell-based (chondrocyte, synoviocyte and leukocyte) and measurement of autoimmune antibodies in sera. In 1996, he established a company that develops and manufactures reagents and kits dedicated to arthritis research. These include highly purified collagen for the CIA model and kits to measure antibodies to type II collagen. The company also developed a rapid model of CIA which is induced by mAbs to type II collagen. He has consulted for many companies and assisted them with their pre-clinical drug discovery efforts.
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arthritis

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rheumatoid arthritis

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osteoarthritis

Expert's expertise lies in the immuno- and pathobiology of cartilage and diseases of cartilage degeneration such osteo- and rheumatoid arthritis. He also has expertise in the isolation and culture of cartilage chondrocytes and measurement of cartilage components such as type II collagen and proteoglycan. In 1996, He established a company that develops and manufactures reagents and kits dedicated to arthritis and cartilage research. These include highly purified collagen for the CIA model and kits to measure type II collagen and antibodies to type II collagen. The company also developed a rapid model of CIA which is induced by mAb to type II collagen. He has consulted for many companies and assisted them with their pre-clinical drug discovery efforts for arthritis and cartilage regeneration.
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cartilage

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collagen

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cartilage disease

Expert has expertise in LPS bioactivity and structure determination from gram-negative bacteria especially those that induce diseases such as periodontal disease (Porphyromonas gingivalis; Pg) and sepsis (E. coli). His research in this area focused on the differential innate host defense response to these LPSs. For example, E. coli (Ec) consists of a canonical hexa-acyl fatty acid structure and elicits a potent response from the innate host defense system. Whereas, Pg has a unique and heterogeneous LPS structure consisting of tri, tetra and penta-acylated fatty acid chains which are poorly recognized by host cells presumably allowing the bacteria to colonize in the periodontal cavity ultimately leading to inflammation and bone loss. To investigate the structure/function relationship of Pg and E. coli LPS, Expert has employed various in vitro cell based assays including E-Selectin and IL-8 expression by endothelial cells and TNF-alpha expression by leukocytes stimulation with LPS. He also developed an in vivo model of LPS stimulation to examine in situ activation of the innate host defense system. Expert also has expertise in biochemical characterization of LPS by employing various techniques including MALDI-TOF gas chromatography and GC/MS.
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lipopolysaccharide

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periodontal disease

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collagen research


Show Secondary and Basic Areas of Expertise
Localities:
Expert may consult nationally and internationally, and is also local to the following cities: Seattle, Washington;  Tacoma, Washington;  Bellevue, Washington;  Everett, Washington;  Federal Way, Washington;  Kent, Washington;  Yakima, Washington;  Bellingham, Washington;  Lakewood, Washington;  and Renton, Washington.

Education:
Year   Degree   Subject   Institution   Honors
1991   Ph. D.   Arthritis Research and Cartilage Biology   University of Tennessee, Memphis  
1984   B. S.   Biomedical Photography   Rochester Institute of Technology   Honors
1980   High School Diploma   pre-college   Toms River High School North   Honor Society Member, top 10% of class

Work History:
Years   Employer   Department   Title   Responsibilities

2000 to

 

(Undisclosed)

 


 

President

 

Expert does consulting for clients in inflammation and arthritis research.

1996 to 2000

 

Chondrex, Inc.

 


 

President and Co-Founder

 

He co-founded the company, which specializes in the manufacture of reagents and contract services for arthritis research.

1994 to 1996

 

Bristol-Myers Squibb

 

Inflammation

 

Post-Doctoral Fellow

 

Expert investigated various diseases including periodontal disease, arthritis, inflammatory bowel disease, and asthma.

1992 to 1993

 

University of Washington

 

Dept. of Orthopaedics

 

Post-Doctoral Fellow

 

He investigated a novel type of collagen in cartilage development and arthritis.

1986 to 1991

 

University of Tennessee

 

Anatomy and Medicine

 

Graduate Student

 

He investigated the mechanisms of collagen-induced arthritis, which is a model of human rheumatoid arthritis.

1984 to 1986

 

NOVARTIS (formerly CIBA-GEIGY AG)

 

Inflammation

 

Research Specialist

 

He investigated methods of digital-imaging processing of radiographs as a non-invasive tool to evaluate arthritis in animal models of human disease.

1982 to 1984

 

Rochester Institute of Technology

 

Biomedical Photographic Communications

 

Lab/Teaching Assistant

 

He assisted professors and maintained the biomedical photographic laboratory at RIT.


Career Accomplishments:
Associations/Societies

Expert is a member of the American College of Rheumatology and Sigma Xi.

Professional Appointments

He is currently a scientific advisor for a biotech company, and formerly was on the board of directors for a gene chip software company and formerly was a scientific advisor for another biotech company.


Publications:
Publications and Patents Summary

Expert has 18 publications, 22 abstracts presented at meetings, two patents submitted, and two book chapters.

Selected Publications and Publishers  
 - Journal of Rheumatology  
 - Developmental Dynamics  
 - Arthritis and Rheumatism  

Government Experience:
Years   Agency   Role   Description
1992 to 1993   Veterans Administration   Post-Doctoral Fellow   Expert worked on the role of a novel alternatively spliced type II collagen mRNA in chondrogenesis and osteoarthritis.

Consulting Services:
Selected Consulting Examples:
  • At a Seattle biotechnology company, Expert worked as a consultant to establish animal models of disease including arthritis, asthma, and multiple sclerosis. These models were used for discovery of novel biotherapeutics.
  • At a Seattle contract research laboratory, he established animal models of human rheumatoid and osteoarthritis. These models were conducted as a service to biotechnology and pharmaceutical companies who wanted to test their experimental therapies.
  • Expert consults for a biotechnology company developing an anti-infective therapy. He tested serum samples from animal models for inflammatory mediators by ELISA.
  • At a Seattle biotechnology company, he worked as a consultant for the pre-clinical evaluation of a biologic for cartilage regeneration. He assisted in establishing animal models of osteo- and rheumatoid arthritis and in vitro chondrocyte assays.
  • Expert consulted for a European CRO/reagent company to establish animal models of disease and develop new reagents/tests for the area of inflammation research.
Recent Client Requests:
  • Expert in rheumatoid arthritis for consulting on RA animal models and compound preclinical development.
  • Expert in laboratory selection for consulting on laboratory selection for collagen induced arthritis CRO.
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Recent Litigation Client Requests:
  • Expert in Trade Secret issue involving Achcia and scutillaria.
  • Expert in The effects of certain supplements on the treatment of osteoarthritis and periodontal disease.
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.

International Experience:
Years   Country / Region   Summary
2003 to 2005   Switzerland   For a European reagent manufacturer and CRO, Expert assisted with the development of human arthritis, asthma, and multiple sclerosis models to provide as a service for biotechnology and pharmaceutical companies. Expert also designed and tested research reagents and kits to be sold to the biomedical community. In addition, he helped to troubleshoot the client's projects.

Market Research:
He co-founded and was president of Chondrex, Inc., a company that manufactures reagents and kits specialized for the area of arthritis research. These are sold to universities and biotechnology and pharmaceutical companies.

Click the green button above to contact Expert for a free initial screening call regarding your marketing research, industry research, and company research needs.  For research needs involving multiple experts or secondary research, a Research Director can be assigned to coordinate the work into a custom report for you as a potentially quicker and more cost effective alternative to doing this work yourself or hiring a boutique consultancy.  Remember, your initial screening call to speak with Expert is free.

Additional Skills and Services:
Training/Seminars

Expert worked as a mentor in an academic institution for many researchers at all levels from undergraduate and graduate students to clinicians.


 

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