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 Expert  723113

Expert in Biological Staining, Fluorescent Probes, Selective Uptake into Live Cells

Available for your Consulting and Expert Witness Needs

United Kingdom
Education Work History Career AccomplishmentsPublicationsConsulting Services Expert Witness

Summary of Expertise:

Listed with other top experts in: 

Expert has a wide-ranging knowledge of, and practical experience with, dyes used in biomedicine – many of which were originally used industrially, eg as textile or food dyes. He has produced novel biological staining applications making use of such dyes; devised analytical procedures for many of them; and prepared trouble-shooting schemes for those using more widely applied dyes.

Novel staining procedures – Expert has shown that new staining procedures, often simpler and more reliable, can be dveloped. For instance those using fuchsine, with fixed tissues. More recently he has developed expertise in assessing which dye structures will result in particular intravellular localization within living cells. The underlying models have also proved applicable to xenobiotics in general, such as drugs, herbicides and pollutants.

Analytical procedure – He has devised widely applicable methods, which for instance can be applied to most acid (ie anionic) and basic (ie cation), mordant (ie dye lakes and metallized), neutral and solvent dyes in general use; as well as to many direct dyes and fluorescent brightening agents.

Trouble shooting – Schemes to trouble-shoot many histopathological stains have been clarified.




acid dye


anionic dye


basic dye


cationic dye


direct dye


fluorescent whitening agent




lake (dye)


metallized dye


mordant dye


neutral dye


solvent dye


azo dye


carmine lake


leuco dye




reactive dye

Cell biology – involves an integrated understanding of the structure (anatomy) and function of biological cells. Expert has considerable experience with interpreting images of a wide variety of cell types [animal, plant and microbial] using a range of microscopic methods and staining reagents. This has proved valuable to specialists first involved with applying a variety of xenobiotics to biological specimens, and using microscopy to assess outcomes.

cell biology


cell (biology)


cell anatomy

Show Secondary and Basic Areas of Expertise

Year   Degree   Subject   Institution   Honors
1961   BSc   Chemistry   University of Sheffield   1st Class Honours
1964   PhD   Physical organic chemistry   University of Sheffield  

Work History:
Years   Employer   Department   Title   Responsibilities

2000 to


(Undisclosed University)


Div of Integrated Biology


Honorary Senior Research Fellow


He carries out biomedical research.

1989 to 1996


University of Sheffield


Dept of Biomedical Science




He engaged in research, teaching and administration within the Department; also helped run an in-house consultancy "Datascope Services".

Career Accomplishments:

Biological Stain Commission National Society of Histotechnology Royal Microscopic Society Scottish Microscopy Group

Publications and Patents Summary

He has more than 150 peer reviewed research papers, 4 major books, and a wide variety of minor publications --- mostly in the field of interaction of dyes and fluorochromes with biological specimens alive and dead.

Selected Publications and Publishers  
 - Biotechnic & Histochemistry  
 - European journal of biology  
 - Elsevier: Edinburgh.  
 - BIOS Scientific Publishers  
 - Churchill Livingstone  

Consulting Services:
Selected Consulting Examples:
  • By inspecting the chemical structures of a set of novel fluorochromes, & estimating their intracellular localization patterns using QSAR models, he could recommend which were most suitable as cell and molecular biological fluorescent probes.
  • He suggested colored labels for young of marine animals of commercial significance, to assess reproductive efficacy under various circumstances.
  • He devised an in-house trouble-shooting guide for a laboratory engaged in the biological staining of a specific type of animal biopsy.
  • He inspected chemical structures of proposed novel pharmaceutical agents, and provided predictions [based on QSAR models] of where they would localise in the appropriate cell type, thus permitting synthesis of the more promising structures.
  • He provided analytical protocols and criteria to permit a biological stain vendor to decide if certain dyes available to him would be of suitable quality for use as biological stains.
Recent Client Requests:
  • Physical chemistry expert for consulting on post-mortem fluorescence of bone.
  • Leuco dye expert for consulting on pigment formulation.
  • Expert in Ink or Dye for alternative material selection.
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Recent Litigation Client Requests:
  • Expert for consulting on R - v - Tomecki.
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.

International Experience:
Years   Country / Region   Summary
2006 to   USA   He is advising which dyes might be most usefully synthesised for labeling a specific biological target.
2001 to 2001   Germany   He devised a trouble-shooting guide for a reagent supplier, for circulation amongst their customers.

Additional Skills and Services:

Recently he has run short courses concerning with predicting the intracellular localisation of xenobiotics [eg drugs, dyes, herbicides] at the New University of Ireland (Galway) and North Eastern (USA). When employed by the University of Sheffield he organised and taught on a variety of undergraduate & graduate school courses in biomedicine.

Other Skills and Services

Predicting the uptake and intracellular localisation of small-molecule xenobiotics [eg drugs, dyes, fluorochromes, herbicides] in living cells [human, animal, plant, microbial] from a knowledge of the xenobiotics chemical structure.


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