The expert's private consulting and research company was established to provide innovative solutions to technical problems for researchers in academia and industry. Its charter is to develop strategies and protocols that streamline scientific research in the most time/cost effective ways.
Research Scientist/Consultant Services:
-Developed nucleic acid assay/detection system—compact, portable (for in-field use)—for identification of infectious diseases or genomic dispositions.
-Reviewed/reported American Red Cross Biomedical Services SOP's in accordance with the Consent Decree (on 04/15/2003) between the American Red Cross (ARC) and the Food and Drug Administration—administered by Quintiles Consulting, Inc.
-Validated genotyping assays. Developed real-time PCR diagnostic assays for EraGen Biosciences, Inc.
-Formulated and validated novel proprietary/system for sequencing of genomic DNA for Genome Technologies, Inc.
-Examined and developed critical QC assays for PowderJect Vaccines, Inc.
-Developed/standardized assays for evaluating Pfu DNA polymerase for Promega Corporation.
-Reformulated buffer/enzyme system for Access RT- PCR System for Promega Corporation.
-Developed technology for automated sample preparation (for use in nucleic acid analysis). Sold rights to BioRad.
-Produced fluorescent/capture oligonucleotide probes and target sequences for automated instrument for Spherotech, Inc.
-Assisted in the development of rapid, colorimetric assay for detection of Chlamydia for Pacific Biotech, Inc.
-Assisted in instrument integration of nucleic acid probe assays for HIV1 and Hepatitis B for Chiron Corp.
Expert's Scientific and Technical Skills:
Sample processing/preparation--tissue cultures, bacterial cultures, animal tissues and body fluids including human;
Nucleic acid amplification-- PCR, RT-PCR, Real-time PCR, QPCR, 3SR/TAS (NASBA), LCR, etc;
DNA sequencing--radioisotopic, automated, etc;
Tissue culture maintenance;
Immunoassay--ELISA/EIA, radioisotopic, agglutination, etc;
Purification methodologies--gel filtration, FPLC, HPLC, affinity chromotagraphy, extractions, etc;
Nucleic acid analysis--western blot, southern blot, northern blot;
Detection technologies--chemiluminescent, fluorescent, colorimetric;
Organic chemistries--nucleic acid, enzyme, ligand, solid-phase conjugations;
-Spreadsheet--Lotus 123/macros, Excel, Quatro Pro;
-Word processing--MSWord, WordPerfect, others;
-Nucleic Acid Analysis--GCG programs, Primer Designer, Oligo, others;
-Sequencing--ABI Software (Navigator w/, Factura w/, AutoAssembler)