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 Expert  724799

Expert in Genomics, Bioinformatics and Molecular Biology


Available for your Consulting and Expert Witness Needs

California (CA)
USA
Education Work History Consulting Services Expert Witness

Summary of Expertise:

Listed with other top experts in: 

Has worked in the area of plant tissue culture, plant genetic engineering and genetic transformation using particle bombardment, with crops such as sorghum and pearl millet. Also, constructed cDNA libraries. He is currently genomics and bioinformatics of Pierce's disease in grapes.

Has worked with development of PCR based chromosomal markers for detection and differentiation of several bacterial species such as Bacillus, Xylella fastidiosa and Huanglongbing (Citrus greening) bacterium. Is an expert in mining SSR and Tandem repeats markers from genomic data for developing strain differentiation and diversity analysis studies.

Has good programming knowledge of PHP, PERL, HTML and MYSQL. Development of relational databases. Took part in developing two software packages SCPrimer and Nway comparison and developed online databases. Extensive experience in whole genome alignment programs such as MUMMER, MVA, MAUVE, and tools such as GenomeViz, RepeatMasker and other related software. Experienced in handling large experimental and bioinformatics data sets and mining of data from various public repositories. DNA sequencing, processing and contig generation using CAP3, LUCY and other related software. Experience in designing and test primers in all popular formats. Knowledge of using a wide variety of computer software for phylogenomics (MEGA and others) and bioinformatics tools.

Experienced in using different gene isolation techniques like RT-PCR, RACE, and magnetic bead -clone capture. Construction and maintenance of cDNA libraries. Real-time quantitative RT-PCR Conducted (using Cephid and ABI-7900 HTP system, Agilent analysis) for functional characterization of the isolated P450 monooxygenase genes. Cloning of the cDNAs into Aspergillus niger expression vectors. Routine handling of protoplast preparation and transformation. Cloning into E.coli vectors and conducting basic expression studies.

Established in vitro plant regeneration systems from a variety of explants like immature inflorescence, immature embryo, shoot tip and shoot meristems of sorghum and Pearl Millet.

Worked on genetic transformation of sorghum using Bt gene constructs, assay of transformants for studying integration, expression and inheritance of transgenes.

Designed oligos for custom microarray to study global gene expression in Vitis spps, Xylella fastidiosa, Bacillus anthracis and Phanerochaete chrysosporium. Experience in handling, printing, hybridization and scanning stages of microarrays.

Extensive knowledge in using microarray data analysis software's such as MEV, SAM and gene spring 4.0 and data interpretation. Experience in data integration with Gene ontologies and use of software's like GenMAPP2 ermineJ and Pathway explorer.

Cloning of the cDNAs into Aspergillus niger expression vectors. Routine handling of protoplast preparation and transformation.

Cloning into E.coli vectors and conducting basic expression studies.

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agricultural biotechnology

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applied microbiology

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bioinformatics

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bacillus molecular biology

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biotechnology

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plant molecular biology

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plant biotechnology

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crop genetic engineering

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genetic engineering

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plant genetic engineering

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plant tissue culture

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gene expression

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gene

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genetic sequencing

Has extensive experience in handling whole genome sequence for annotation, detection of sequence variation and development of relational databases.

Has done functional genomic analysis of the Xylella fastidiosa and Vitis spps. interaction using microarray and bioinformatics tools. This includes, generation of transcription profiles for the plant-pathogen interaction and identification and quantification of the micro and macro genomic variability among the different Xylella isolates and correlate it with the pathogen virulence.

for the biothreat agents Bacillus anthracis and Francisella tularensis, using MUMMER and such other bioinformatics software's, a whole genome search for SNPs, INDELs and similar other variable regions was conducted for the above two biothreat agents. Multiplex PCR based assay has been standardized for B. anthracis and screening of these primers across other Bacillus spp has been completed. Similarly, genome-wide search was conducted for STRs (short tandem repeats) in the above two genomes.

Is an expert in designing oligos primers across phyla and for several molecular biology experiments such as gene cloning, species detection and strain differentiation. has developed software tools for designing PCR primers.

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genome

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polymerase chain reaction


Show Secondary and Basic Areas of Expertise
Localities:
Expert may consult nationally and internationally, and is also local to the following cities: Fresno, California;  Bakersfield, California;  Salinas, California;  Visalia, California;  Clovis, California;  Merced, California;  and Turlock, California.

Education:
Year   Degree   Subject   Institution  
2004   PhD   Genetics   Osmania University, Hyderabad, India  
1994   M.Sc   Plant Sciences   University of Hyderabad  
1992   B.Sc   Botany Zoology Chemistry   Osmania University, India  

Work History:
Years   Employer   Department   Title   Responsibilities

2005 to

 

(Undisclosed University)

 

Viticulture and Enology

 

Postdoc employee

 

He is working on genomics and Bioinformatics projects related the Pierce's disease of grapewine caused by the bacterium Xylella fastidiosa

2002 to 2005

 

University of Cincinnati

 

Environmental Health

 

Visiting Scholar

 

He was working on the Genomics, molecular biology and Bioinformatics of the wood rotting fungus phanerochaete chrysosporium.


Government Experience:
Years   Agency   Role   Description
2005 to   USDA-ARS, PARLIER, CA   Visiting Scientist   He is working on several bioinformatics related projects in the Crops pests disease and genetics (CDPG) unit as a part of the specific collaborative agreement with the UC, Davis.

Consulting Services:
Selected Consulting Examples:
  • He established in vitro plant regeneration systems from a variety of explants like immature inflorescence, immature embryo, shoot tip and shoot meristems of sorghum. He worked on genetic transformation of sorghum using Bt gene constructs, assay of transformants for studying integration, expression and inheritance of transgenes.
  • He is an expert in designing PCR primers and oligos for custom microarray to study global gene expression in Vitis spps, Xylella fastidiosa, Bacillus anthracis and Phanerochaete chrysosporium. Experience in handling, printing, hybridization and scanning stages of microarrays. He has extensive knowledge in using microarray data analysis software's such as MEV, SAM and gene spring 4.0 and data interpretation. Experience in data integration with Gene ontologies and use of software's like GenMAPP2 ermineJ and Pathway explorer.
  • He has good programming knowledge of PHP, PERL, HTML and MYSQL. Development of relational databases. comparative genomics, identifying specific DNA biomarkers for detection and differentiation of bacteria and fungi and other pathogens.
  • He has Knowledge of using a wide variety of computer software for phylogenomics and bioinformatics tools EST and genomic sequence analysis.
Recent Client Requests:
  • Expert in new product development to provide input into launch of disruptive qPCR technology.
Click the green button above to contact Expert for a free initial screening call regarding your expert consulting needs.  Expert is available for consulting to corporate, legal and government clients.  Remember, your initial screening call to speak with Expert is free.

Expert Witness:
Click the green button above to contact Expert for a free initial screening call regarding expert testimony, litigation consulting and support, forensic services, or any related expert witness services.  A few litigation needs include product liability, personal injury, economic loss, intellectual property (patent, trademark, trade secret, copyright), and insurance matters.  Remember, your initial screening call to speak with Expert is free.

Language Skills:
Foreign Language   Description
Hindi, Telugu   He is an expert in Hindi (Indian national language) and Telugu (popular south Indian language). Can handle consulting services in these two languages apart from English.

 

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